Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
Subcellular Location:
Rough endoplasmic reticulum
Nucleus membrane
Golgi apparatus
Cytoplasm
perinuclear region
Secreted
Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Monoclonal Antibody for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
Protein for Bovine Prostaglandin-H2 D-isomerase
R&D Technical Data
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s
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.3%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of PTGDS into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
98
92-102
Plasma
99
96-104
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGDS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
92-103%
92-104%
94-102%
EDTA plasma(n=5)
91-106%
93-105%
96-101%
95-100%
heparin plasma(n=5)
94-101%
91-104%
95-102%
93-105%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 29-48;CATALYTIC ACTIVITY;TISSUE SPECIFICITY
Vaild Sequence:
